全文获取类型
收费全文 | 89440篇 |
免费 | 4943篇 |
国内免费 | 5756篇 |
出版年
2023年 | 1028篇 |
2022年 | 1156篇 |
2021年 | 2672篇 |
2020年 | 1953篇 |
2019年 | 2517篇 |
2018年 | 2110篇 |
2017年 | 1590篇 |
2016年 | 2282篇 |
2015年 | 4033篇 |
2014年 | 7413篇 |
2013年 | 7239篇 |
2012年 | 5713篇 |
2011年 | 6257篇 |
2010年 | 4683篇 |
2009年 | 4357篇 |
2008年 | 4527篇 |
2007年 | 4976篇 |
2006年 | 3613篇 |
2005年 | 3153篇 |
2004年 | 2328篇 |
2003年 | 2016篇 |
2002年 | 1906篇 |
2001年 | 1541篇 |
2000年 | 1345篇 |
1999年 | 1267篇 |
1998年 | 1050篇 |
1997年 | 830篇 |
1996年 | 957篇 |
1995年 | 1025篇 |
1994年 | 925篇 |
1993年 | 942篇 |
1992年 | 883篇 |
1991年 | 871篇 |
1990年 | 746篇 |
1989年 | 685篇 |
1988年 | 658篇 |
1987年 | 526篇 |
1986年 | 494篇 |
1985年 | 783篇 |
1984年 | 1030篇 |
1983年 | 663篇 |
1982年 | 873篇 |
1981年 | 849篇 |
1980年 | 635篇 |
1979年 | 610篇 |
1978年 | 408篇 |
1977年 | 415篇 |
1976年 | 366篇 |
1974年 | 268篇 |
1973年 | 278篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Sophie J. Milward Natalie Sebanz 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2016,371(1686)
This opinion piece offers a commentary on the four papers that address the theme of the development of self and other understanding with a view to highlighting the important contribution of developmental research to understanding of mechanisms of social cognition. We discuss potential mechanisms linking self–other distinction and empathy, implications for grouping motor, affective and cognitive domains under a single mechanism, applications of these accounts for joint action and finally consider self–other distinction in group versus dyadic settings. 相似文献
102.
In the non-heterocyst, marine cyanobacterium Trichodesmium nitrogen fixation is confined to the photoperiod and occurs coevally with oxygenic photosynthesis although nitrogenase is irreversibly inactivated by oxygen. In previous studies it was found that regulation of photosynthesis for nitrogen fixation involves Mehler reaction and various activity states with reversible coupling of photosynthetic components. We now investigated these activity states in more detail. Spectrally resolved fluorescence kinetic measurements of single cells revealed that they were related to alternate uncoupling and coupling of phycobilisomes from and to the photosystems, changing the effective cross-section of PSII. Therefore, we isolated and purified the phycobiliproteins of Trichodesmium via ion exchange chromatography and recorded their UV/VIS absorption, fluorescence excitation and fluorescence emission spectra. After describing these spectra by mathematical equations via the Gauss-Peak-Spectra method, we used them to deconvolute the in vivo fluorescence spectra of Trichodesmium cells. This revealed that the contribution of different parts of the phycobilisome antenna to fluorescence quenching changed during the daily activity cycle, and that individual phycobiliproteins can be reversibly coupled to the photosystems, while the expression levels of these proteins did not change much during the daily activity cycle. Thus we propose that variable phycobilisome coupling plays a key role in the regulation of photosynthesis for nitrogen fixation in Trichodesmium. 相似文献
103.
Masood Badri Guang Yang Karima Al Mazroui Jihad Mohaidat Asma Al Rashedi Najwa Al Housani 《Journal of biological education》2017,51(2):166-185
This study employed the international Relevance of Science Education questionnaire to survey the interest in biology and the out-of-school experiences of Abu Dhabi secondary school students (median age 17, mean age 17.53 and mode age of 16) in the third semester of 2014. It included 3100 participants. An exploratory factor analysis was used to categorise the items for both interest in biology and out-of-school experience. Ten interest in biology and 12 out-of-school experience factors were extracted. The summated means for each factor indicated that ‘health and fitness’ and ‘disease control’ enjoyed highest interests among students. For out-of-school experiences, the two factors of ‘digital applications’ and ‘medical treatment’ received the highest scores. Multivariate analysis of variance revealed that all factors for both interest in biology and out-of-school experience exhibited significant differences between boys and girls. More girls than boys were interested in disease control, reproduction (human biology), alternative science, health and fitness, zoology, and applied cosmetic biology. No significant differences were observed for the remaining five other categories. Furthermore, analysis of variance revealed significant differences between boys and girls with regard to individual items comprising each of the factors. The highest correlations were between the two factors of out-of-school experiences of ‘the natural world’ and ‘learning through observation’ and the interest in biology factor related to ‘plant and animal farming and agriculture’. Results suggested that more emphasis must be placed on students’ out-of-school experience and their engagement in informal learning in contextual outdoor environments to enhance their interest in learning more about biology and the living environment in general. 相似文献
104.
Respiration, which is the second most important carbon flux in ecosystems following gross primary productivity, is typically represented in biogeochemical models by simple temperature dependence equations. These equations were established in the 19th century and have been modified very little since then. Recent applications of these equations to data on soil respiration have produced highly variable apparent temperature sensitivities. This paper searches for reasons for this variability, ranging from biochemical reactions to ecosystem‐scale substrate supply. For a simple membrane‐bound enzymatic system that follows Michaelis–Menten kinetics, the temperature sensitivities of maximum enzyme activity (Vmax) and the half‐saturation constant that reflects the affinity of the enzyme for the substrate (Km) can cancel each other to produce no net temperature dependence of the enzyme. Alternatively, when diffusion of substrates covaries with temperature, then the combined temperature sensitivity can be higher than that of each individual process. We also present examples to show that soluble carbon substrate supply is likely to be important at scales ranging from transport across membranes, diffusion through soil water films, allocation to aboveground and belowground plant tissues, phenological patterns of carbon allocation and growth, and intersite differences in productivity. Robust models of soil respiration will require that the direct effects of substrate supply, temperature, and desiccation stress be separated from the indirect effects of temperature and soil water content on substrate diffusion and availability. We speculate that apparent Q10 values of respiration that are significantly above about 2.5 probably indicate that some unidentified process of substrate supply is confounded with observed temperature variation. 相似文献
105.
Inter simple sequence repeat (ISSR) marker assay was employed to validate the genetic fidelity of Swertia chirayita plantlets multiplied in vitro by axillary multiplication upto forty-two passages. Sixteen ISSR primers generated a total of 102 amplicons among the tissue-cultured
plants. Forty-eight amplicons were amplified in the outlier (a Swertia species). The outlier (negative control) was employed to rule out the possibility that the invariant fingerprint was due
to chance alone and that the ISSR technique employed was not discriminatory enough to detect the off-types. A homogenous amplification
profile was observed for all the micropropagated plants. The results confirmed the clonal fidelity of the tissue culture-raised
S. chirayita plantlets and corroborated the fact that axillary multiplication is the safest mode for multiplication of true to type plants. 相似文献
106.
Effects of facilitation on community stability and dynamics: synthesis and future directions 总被引:1,自引:1,他引:0
Bradley J. Butterfield† 《Journal of Ecology》2009,97(6):1192-1201
107.
RAPHAEL LUGAN MARIE-FRANÇOISE NIOGRET LUCIE KERVAZO FRANÇOIS ROBERT LARHER JOACHIM KOPKA & ALAIN BOUCHEREAU 《Plant, cell & environment》2009,32(2):95-108
Metabolomic investigation of the freezing-tolerant Arabidopsis mutant esk1 revealed large alterations in polar metabolite content in roots and shoots. Stress metabolic markers were found to be among the most significant metabolic markers associated with the mutation, but also compounds related to growth regulation or nutrition. The metabolic phenotype of esk1 was also compared to that of wild type (WT) under various environmental constraints, namely cold, salinity and dehydration. The mutant was shown to express constitutively a subset of metabolic responses which fits with the core of stress metabolic responses in the WT. But remarkably, the most specific metabolic responses to cold acclimation were not phenocopied by esk1 mutation and remained fully inducible in the mutant at low temperature. Under salt stress, esk1 accumulated lower amounts of Na+ in leaves than the WT, and under dehydration stress its metabolic profile and osmotic potential were only slightly impacted. These phenotypes are consistent with the hypothesis of an altered water status in esk1 , which actually exhibited basic lower water content (WC) and transpiration rate (TR) than the WT. Taken together, the results suggest that ESK1 does not function as a specific cold acclimation gene, but could rather be involved in water homeostasis. 相似文献
108.
109.
110.